Abstract: Objective To investigate the current status of occupational burnout and sleep quality among maternal and child healthcare(MCH) workers, examine their relationship, and analyze influencing factors of sleep quality. Methods In September 2024, a convenience sampling method was used to recruit 404 MCH workers in a city. They were surveyed with the self-designed demographic questionnaire, the Maslach Burnout Inventory-General Survey(MBI-GS), and the Pittsburgh Sleep Quality Index(PSQI). Multiple linear regression analysis was conducted to identify factors affecting sleep quality. Results A total of 392 valid questionnaires were collected, with an effective response rate of 97.03%. The incidence of occupational burnout was 64.29%, and the incidence of sleep disturbances was 48.98%. Higher total burnout scores were observed among clinical staff, middle-level and senior managers, personnel from dis-rict/county-level institutions, and personnel with low work interest, higher overtime frequency, greater work pressure, poorer departmental harmony, and poorer health status (all P < 0.05). Scores across all dimensions of occupational burnout were positively correlated with scores across all dimensions of sleep quality (r = 0.123 to 0.556, all P < 0.05). Multiple linear regression analysis showed that compared with personnel in poorer health status, those with fair or good health status had average decreases of 2.130 and 4.433 points in sleep quality scores, respectively (both P < 0.05). Each 1-point increase in occupational burnout score was associated with a 0.627-point increase in sleep quality score (P < 0.05). Conclusions Maternal and child healthcare workers in the surveyed city experienced varying degrees of occupational burnout and generally poor sleep quality. Occupational burnout was positively correlated with sleep quality. Targeted management measures should be taken to alleviate burnout and improve sleep quality among these healthcare workers.