Abstract: Objective To study the positive effects of the new type orientation movement-based sports and health courses on physiological function of students majoring in first-aid, so as to explore different approaches to cultivating high quality first-aid professionals. Methods A total of 60 students majoring in first-aid were classified into experimental group who received orientation movement-based education, and 55 students majoring in clinical medicine were classified into control group who received traditional model physical education. On Monday, Wednesday and Friday of each week, the students attended different models of physical classes for 90 minutes. This course continued for a semester (19 weeks), and the other courses still followed routine practices as scheduled. Before and after the experiment, test was conducted on physical constitution, physical ability, emergency treatment skills and relevant indexes of the two groups of students and statistical analysis was made. Results The step exponent, vertical jump in situ and one-time CPR of the experimental group were improved significantly after the experiment (t=-9.564,-0.933,-7.046,P<0.05), while the control group showed no significant differences (P>0.05) though the values of various indexes showed improvement to some extent after the experiment. The various muscle mass indexes except for body mass index and the left arm muscle mass, step exponent, body forward bending, vertical jumping in situ and time spent in implementation of CPR of the experiment group students showed significantly better than those of the control group(t =-2.436,2.323,2.356,2.273,1.987,6.603,2.085,6.241,P<0.05 or 0.01). Conclusion Orientation movement was helpful to train students majoring in first-aid, and of great theoretical significance and application value.