摘要:
目的 建立尿中锡的湿法消解-氢化物发生-原子荧光光谱测定法。 方法 尿样经v (硝酸):v (硫酸)为40:1的混合酸消化后,加入50 g/L硫脲+50 g/L抗坏血酸为掩蔽剂,以体积分数为0.5%的硫酸为载流,以10.0 g/L硼氢化钾为还原剂,采用氢化物发生-原子荧光光谱法测定尿锡浓度。 结果 在10.0~100.0μg/L的线性范围内,尿锡测定所得回归方程为y=22.847 x+26.395,相关系数为0.999 7。该方法的检出限为0.33μg/L,RSD为0.96%~2.64%,回收率在97.27%~101.30%之间。 结论 该方法具有简单快速、准确度和灵敏度高、基体干扰少的优点,所用原子荧光仪器相对便宜,适合基层单位对尿锡质量浓度的测定。
+ English
Abstract:
Objective To establish a method for the determination of tin in urine by wet digestion hydride generation -atomic fluorescence spectrometry. Methods After the samples were digested with the acid mixed with nitric acid and sulfuric acid(40:1,volume ratio), 50μg/L thiourea-50μg/L ascorbic acid were added in it as a masking agent and 10.0 g/L potassium borohydride were added as reducing agent. The determination of tin concentration in urine was done by hydride generation-atomic fluorescence spectrometry with 0.5% (volume ratio)sulfuric acid for load flow. Results Within the linear range of 10.0-100.0μg/L, the obtained regression equation was y (the urinary tin concentration)=22.847 x (peak area) + 26.395. The correlation coefficient was 0.999 7. The detection limit was 0.33μg/L;RSDs range from 0.96% to 2.64% and the recovery rates 97.27%-101.30%. Conclusion This method is simple, fast, accurate and sensitive, and the matrix interference is little. As the atomic fluorescence apparatus is relatively cheap, this method is suitable for the determination of urine tin concentration.
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