职业接触铟工人血和尿中铟的检测
Determination of indium in the blood and urine of workers exposed to indium
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摘要:目的 建立血和尿中铟(In)的检测方法, 评估职业接触工人In的接触水平。方法 血样和尿样用体积分数0.1%的硝酸+体积分数0.02%的曲拉通溶液将原体积稀释至10倍后, 采用工作曲线法定量, 利用电感耦合等离子体质谱(ICP-MS)法测定血和尿样中In的质量浓度, 并用该法对某液晶屏生产企业的82名接触In工人血和尿样中In进行检测。结果 血和尿中In在选定范围内均具有良好的线性关系, 相关系数大于0.999, 血和尿中In检出限分别为0.014 5 μg/L和0.005 1 μg/L, 最低检出的质量浓度分别为0.145 μg/L和0.051μg/L, 血和尿中In的加标回收率分别为98.3%~103.2%和95.2%~100.5%, 检测的批内精密度分别为1.11%~2.05%和1.02%~1.99%, 批间精密度分别为2.08%~3.99%和1.14%~1.82%。82名工人尿中In的质量浓度均低于最低检出浓度, 血中In的质量浓度为 < 0.145~1.730 μg/L。结论 建立的方法有检出限低、灵敏度高、精密度好等优点, 可用于In接触工人血和尿中In的检测。Abstract:Objective To establish a determination method for indium in blood and urine.Methods Blood and urine were diluted with the solution 0.1% HNO3 + 0.02% Trion X-100 and detected by ICP-MS. The working curve method was applied for the quantitative determination. The indium in the blood and urine of 82 workers exposed to indium in a LCD manufacturing enterprise was detected with this method.Results The linear relationship in the selected range was good and the related coefficient was greater than 0.999. The detection of limit for the blood and urine were 0.014 5 μg/L and 0.005 1 μg/L, respectively. The minimum detectable analytical concentrations were 0.145 μg/L in blood and 0.051 μg/L in urine. The recovery rate for blood and urine was 98.3%-103.2% and 95.2-100.5%, respectively. The relative standard deviation in batch for blood and urine were 1.11%-2.05% and 1.02% -1.99%, and the relative standard deviation between batch for blood and urine were 2.08%-3.99% and 1.14%-1.82%, respectively. Indium was not detected in the urine of those workers exposed to indium and the concentration of indium in the blood was < 0.145-1.730 μg/L among them.Conclusion This methodology has the advantages of low detect limit, high sensitivity and good precision. It can be used in biological monitoring of workers exposed to indium.
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