谢英, 李智民, 沙焱, 杨新跃, 陈志军, 罗军, 张柏林, 张雄, 纪祥. 二次气管切开灌注二氧化硅构建大鼠硅肺模型的效果观察[J]. 职业卫生与应急救援, 2018, 36(6): 481-484, 496. DOI: 10.16369/j.oher.issn.1007-1326.2018.06.001
引用本文: 谢英, 李智民, 沙焱, 杨新跃, 陈志军, 罗军, 张柏林, 张雄, 纪祥. 二次气管切开灌注二氧化硅构建大鼠硅肺模型的效果观察[J]. 职业卫生与应急救援, 2018, 36(6): 481-484, 496. DOI: 10.16369/j.oher.issn.1007-1326.2018.06.001
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XIE Ying, LI Zhiming, SHA Yan, YANG Xinyue, CHEN Zhijun, LUO Jun, ZHANG Bolin, ZHANG Xiong, JI Xiang. Rat pneumoconiosis induced by tracheal infusion of SiO2 twice after tracheotomy[J]. Occupational Health and Emergency Rescue, 2018, 36(6): 481-484, 496. DOI: 10.16369/j.oher.issn.1007-1326.2018.06.001
Citation: XIE Ying, LI Zhiming, SHA Yan, YANG Xinyue, CHEN Zhijun, LUO Jun, ZHANG Bolin, ZHANG Xiong, JI Xiang. Rat pneumoconiosis induced by tracheal infusion of SiO2 twice after tracheotomy[J]. Occupational Health and Emergency Rescue, 2018, 36(6): 481-484, 496. DOI: 10.16369/j.oher.issn.1007-1326.2018.06.001
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二次气管切开灌注二氧化硅构建大鼠硅肺模型的效果观察

Rat pneumoconiosis induced by tracheal infusion of SiO2 twice after tracheotomy

    摘要
  • 摘要:
    目的 通过二次气管切开灌注二氧化硅建立硅肺动物模型, 为探讨硅肺病发病机制、寻找更有效的治疗方法提供模型支持。
    方法 将40只SD雄性大鼠随机分为对照组和模型组各20只。模型组采用二次(间隔7 d)气管切开灌注0.5 mL SiO2混悬液(50 mg/mL)建立硅肺大鼠模型, 对照组通过气管滴注注入生理盐水。两组均在第一次染尘90 d后行CT检查, 并于处死后进行HE和Masson染色观察肺组织病理学变化; 用酶联免疫法检测大鼠血清白介素-6(IL-6)和转化生长因子β1(TGF-β1)的水平, 酸解法检测肺组织羟脯胺酸(HYP)水平的变化。
    结果 模型组大鼠肺组织双肺表面可见散在的灰白色斑点, 部分融合成不规则形斑块; CT摄影可见肺部呈弥漫性云雾状片状高密度影; 病理检查结果显示肺组织出现细胞性硅结节(肉芽肿)等病理改变; 模型组以上影像学、大体外观、组织学病理改变各有16例、20例、20例, 而对照组均未发生改变。模型组IL-6、HYP、TGF-β1水平较对照组均明显升高, 组间差异均有统计学意义(P < 0.01)。
    结论 采用二次染尘法气管内二次注入二氧化硅(SiO2)建立硅肺大鼠模型, 能够达到满意效果。

     

    Abstract:
    Objective To establish the pneumoconiosis model through twice-tracheal infusion of SiO2 in rats after tracheotomy and to provide the model for exploring the pathogenesis of silicosis and therapeutic methods.
    Methods Totally 40 healthy male SD rats were randomly divided into 2 groups, namely model and control groups. The rats in model group were received tracheal infusion of silica crystals (SiO2, 50 mg/mL) for two times (7 days interval), while the rats in control group were received saline. On day 90, all rats were underwent CT scans. And on next day, all rats were executed and the pathologic changes in lung tissues of rats were observed with HE and Masson staining. The changes of IL-6, TGF-β1 in serum and HYP of lung tissue were detected by Elisa methods and acid hydrolysis method respectively.
    Results The gross examination showed all rats in the model group had the scattered gray-white spots which were seen on the surface of lungs, and some of them fused into irregular plaques. The CT examination showed 16 rats had the cloudy and streak on high-frequency shade. Pathological examination showed all rats had celluloid nodules (granuloma) in lung tissue. Correspondingly, there were no changes noted in the rats in the control group. The IL-6, HYP and TGF-β1 levels of rats in model group were increased compared with those of rats in control group(P < 0.01).
    Conclusion Rat pneumoconiosis could be successfully established through twice-tracheal infusion of SiO2 in rats after tracheotomy.

     

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