优化血液灌流清除毒死蜱及其代谢产物条件的体外实验

郭翔; 李添娣; 谢玉璇; 何俊涛; 刘奋; 何坚; 林辉; 郭美琼

doi:10.16369/j.oher.issn.1007-1326.2019.04.006

优化血液灌流清除毒死蜱及其代谢产物条件的体外实验

Study in vitro on hemoperfusion to eliminate chlorpyrifos and its metabolites

摘要

摘要:
目的利用正交设计和体外实验的方法评价血液灌流清除毒死蜱及其代谢产物的效果。
方法建立体外模拟血液灌流的实验模型。通过正交实验设计探索血药浓度、血液灌流速度、吸附剂的种类和血液灌流持续时间等因素对血液灌流清除毒死蜱及其代谢产物能力的影响。
结果血药浓度、血液灌流速度、吸附剂种类和血液灌流持续时间对毒死蜱及其代谢产物的清除率均有影响（P < 0.01）。对清除率影响程度大小的顺序为血液灌流持续时间>吸附剂种类>血药浓度>血液灌流速度。正交实验表明各因素的最佳组合为在低血药浓度（毒死蜱为1 mg/L，3，5，6-三氯-2-吡啶醇为10 mg/L）中采用2 mL/min血流速度、活性炭吸附剂、血液灌流持续120 min，此时毒死蜱及其代谢产物的清除率最高。
结论体外实验条件下，血液灌流可有效清除毒死蜱及其代谢产物。血液灌流速度、吸附剂的种类、血液灌流持续时间对毒死蜱及其代谢产物的清除率均有影响。

Abstract:
Objective To study the clearance of hemoperfusion in vitro against chlorpyrifos and its metabolites.
Methods The experimental model for hemoperfusion in vitro was established. The effects of plasma concentrate, blood flow velocity, type of adsorbents and perfusion time on the clearance of chlorpyrifos and its metabolites were evaluated by orthogonal experimental design.
Results The orthogonal experimental test showed that all the studied factors, including plasma concentration, blood flow velocity, type of adsorbents and perfusion time, significantly influenced the absorption of chlorpyrifos and its metabolites (P < 0.01). The order of factors affecting the absorption was perfusion time, type of adsorbents, blood toxins concentrate, and blood flow velocity. Orthogonal experiments showed that the best combination was 2 mL/min blood flow rate, activated carbon adsorbent and hemoperfusion time for 120 minutes for low blood concentration of chlorpyrifos (1 mg/L) and its metabolites (3, 5, 6-trichloro-2-pyridinol, 10 mg/L), and the clearance rate of chlorpyrifos and its metabolites was the highest.
Conclusion In vitro test show that hemoperfusion could clear chlorpyrifos and its metabolites. Blood flow velocity, type of adsorbents and perfusion time could influence the absorption of chlorpyrifos.

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