紫外分光光度法测定全血胆碱酯酶活性测定试纸中溴化乙酰胆碱含量方法的建立

许兴兴; 刘艳芹; 李莉; 丁日高; 赵建

doi:10.16369/j.oher.issn.1007-1326.2019.04.016

紫外分光光度法测定全血胆碱酯酶活性测定试纸中溴化乙酰胆碱含量方法的建立

Determination by UV spectrophotometry of acetylcholine bromide content in test-strip for measuring whole blood cholinesterase activity

摘要

摘要:
目的建立紫外分光光度法测定全血胆碱酯酶活性测定试纸（简称测酶试纸）中溴化乙酰胆碱含量的方法，并验证该方法的可行性。
方法利用溴化乙酰胆碱和溴百里香酚蓝（测酶试纸的两种主要成分）的溶解性差异，将测酶试纸中的溴化乙酰胆碱洗脱下来，以浓度为2.8、5.6、8.4、11.2、14.0 mmol/L的溴化乙酰胆碱对照品溶液制作测酶试纸中溴化乙酰胆碱含量测定方法的标准曲线，并对该法的标准曲线线性、准确度、精密度、耐用性及回收率进行验证。
结果该方法的标准曲线线性良好，线性相关系数的平均值为0.999 79。该法的准确度、精密度和耐用性良好，批内和批间相对标准偏差均不超过4%，加样回收率在94.90%~106.22%之间。
结论该方法可用于测定测酶试纸中溴化乙酰胆碱的含量。

Abstract:
Objective To establish an ultraviolet spectrophotometry method for the determination of acetylcholine bromide content in test strip for measuring whole blood cholinesterase activity, and validate the feasibility of this method.
Methods The acetylcholine bromide in the test strip was eluted by using the difference in solubility between acetylcholine bromide and bromothymol blue(the two main components of the test strip). The standard curve of the method was established by using control solution containing 2.8, 5.6, 8.4, 11.2 and 14.0 mmol/L of acetylcholine bromide, and the standard curve linearity, accuracy, precision, durability and recovery of the method were validated.
Results The standard curve of this method was linear and the average of linear correlation coefficients was 0.999 79. The accuracy, precision and durability of the method were good. The Intra- and inter-batch relative standard deviations were both less than 4%, and the recovery rates were between 94.90% and 106.22%.
Conclusion This method could be used for quantitative detection of acetylcholine bromide content in test-strip for measuring whole blood cholinesterase activity.

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