调节性T细胞及调节性B细胞在三氯乙烯药疹样皮炎发病中的作用研究

邓立华; 鲁焱; 邓丽丹; 韦始亮; 梁实

doi:10.16369/j.oher.issn.1007-1326.2020.01.002

调节性T细胞及调节性B细胞在三氯乙烯药疹样皮炎发病中的作用研究

Role of regulatory T cells and regulatory B cells in pathogenesis of occupational medicamentosa-like dermatitis due to trichloroethylene

摘要

摘要:
目的研究三氯乙烯（TCE）药疹样皮炎患者外周血调节性T细胞和调节性B细胞的变化特点。
方法采用流式细胞术，分别于急性期和临床治愈期对15例TCE药疹样皮炎患者进行外周血CD4⁺CD25⁺调节性T细胞（CD4⁺CD25⁺Tregs）及CD19⁺CD24⁺CD27⁺调节性B细胞（CD19⁺CD24⁺CD27⁺Bregs）检测，并与15例不接触TCE的健康对照者进行比较。
结果 TCE药疹样皮炎患者急性期外周血CD4⁺CD25⁺Tregs比值（0.99%±0.55%）及CD19⁺CD24⁺CD27⁺Bregs比值（7.86%±2.35%）均低于对照组（P < 0.01）；临床治愈期CD4⁺CD25⁺Tregs比值（2.54%±0.42%）及CD19⁺CD24⁺CD27⁺Bregs比值（23.28%±8.62%）高于急性期（P < 0.01），但临床治愈期CD4⁺CD25⁺Tregs比值（2.54%±0.42%）仍均低于对照组，以上差异均有统计学意义（P < 0.01）。
结论 TCE药疹样皮炎患者急性期调节性T细胞及调节性B细胞数量明显减少，治疗后随病情好转数量明显增高，提示调节性B细胞可能与调节性T细胞一起参与TCE致药疹样皮炎的致病过程。

Abstract:
Objective To study the characteristics of regulatory T cells and regulatory B cells in peripheral blood of patients with occupational medicamentosa-like dermatitis due to trichloroethylene.
Methods The CD4⁺CD25⁺ regulatory T cells (CD4⁺CD25⁺Tregs) and CD19⁺CD24⁺CD27⁺ regulatory B cells (CD19⁺CD24⁺CD27⁺Bregs) in peripheral blood of 15 patients were detected with flow cytometry at their acute stage and the clinical cure stage, respectively, and were compared with that of 15 healthy controls without any exposure to trichloroethylene.
Results At acute stage, the patients' ratios of CD4⁺CD25⁺Tregs (0.99%±0.55%) and CD19⁺CD24⁺CD27⁺Bregs (7.86%±2.35%) in peripheral blood were significantly decreased compared with that of control persons(P < 0.01). At cured stage, their ratios of CD4⁺CD25⁺Tregs (2.54%±0.42%) and CD19⁺CD24⁺CD27⁺Bregs (23.28%±8.62%)were recovered compared with that at acute stage(P < 0.01), but still lower than that of the control persons(P < 0.01).
Conclusion Regulatory T cells and regulatory B cells of patients were significantly decreased and could be improved after treatment. These indicated that regulatory T cells, regulatory B cells were also involved in the pathogenesis of occupational medicamentosa-like dermatitis due to trichloroethylene.

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