石墨炉原子吸收法测定尿中锰标准方法改进探讨

杨福成; 施丝; 屈亚平; 裴雯; 杜航

doi:10.16369/j.oher.issn.1007-1326.2020.03.022

石墨炉原子吸收法测定尿中锰标准方法改进探讨

Improvement of national standard method for determination of urinary manganese by GFAAS

摘要

摘要:
目的对石墨炉原子吸收光谱法测定尿中锰的标准方法进行改进。
方法以工作曲线法代替标准曲线法，在测定中加入硝酸钯基体改进剂。
结果方法改进后显示，锰浓度在0.0 ~ 12.0 μg/L呈良好线性关系，回归方程为y = 0.007 50 x + 0.000 2（y为吸光度值，x为锰质量浓度），相关系数为0.999 7，检出限为0.12 μg/L，定量下限为0.39 μg/L；相对标准偏差为0.77% ~ 2.15%，回收率为98.6% ~ 105.0%。
结论改进后的方法具有线性好、背景吸收低，精密度、准确度、灵敏度高，峰型好、易操作等优点，适用于尿中锰浓度的测定。

Abstract:
Objective To improve national standard method of graphite furnace atomic absorption spectrometry for determination of manganese in urine.
Methods Palladium nitrate solution was used as chemical modifier and working curve method was performed with matrix matched calibration solutions, instead of standard curve method.
Results The linear relationship was obtained in the manganese concentration range of 0.0-12.0 μg/L. The regression equation was y = 0.007 50 x + 0.000 2 with correlation coefficient (R²) of 0.999 7. The LOD and LOQ were found to be 0.12 μg/L and 0.39 μg/L, respectively. Tests for recovery were made by standard addition method, and ranged from 98.6%-105.0% with RSD in the range of 0.77% - 2.15%.
Conclusion The results obtained suggest that the developed method has the advantages of low background absorption and good shape of peak. It demonstrates a good practical applicability for rapid, sensitive, precise and accurate determination of manganese in urine.

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