王晓月, 陈东真, 梁嘉祺, 杜忠君, 田小雨, 鞠佰芳, 王洪宾, 丁国永. 骨髓间充质干细胞促小鼠皮肤烫伤修复研究[J]. 职业卫生与应急救援, 2020, 38(5): 443-446, 451. DOI: 10.16369/j.oher.issn.1007-1326.2020.05.002
引用本文: 王晓月, 陈东真, 梁嘉祺, 杜忠君, 田小雨, 鞠佰芳, 王洪宾, 丁国永. 骨髓间充质干细胞促小鼠皮肤烫伤修复研究[J]. 职业卫生与应急救援, 2020, 38(5): 443-446, 451. DOI: 10.16369/j.oher.issn.1007-1326.2020.05.002
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WANG Xiaoyue, CHEN Dongzhen, LIANG Jiaqi, DU Zhongjun, TIAN Xiaoyu, JU Baifang, WANG Hongbin, DING Guoyong. Effect of bone marrow mesenchymal stem cells on recovery of skin burn in C57BL/6J mice[J]. Occupational Health and Emergency Rescue, 2020, 38(5): 443-446, 451. DOI: 10.16369/j.oher.issn.1007-1326.2020.05.002
Citation: WANG Xiaoyue, CHEN Dongzhen, LIANG Jiaqi, DU Zhongjun, TIAN Xiaoyu, JU Baifang, WANG Hongbin, DING Guoyong. Effect of bone marrow mesenchymal stem cells on recovery of skin burn in C57BL/6J mice[J]. Occupational Health and Emergency Rescue, 2020, 38(5): 443-446, 451. DOI: 10.16369/j.oher.issn.1007-1326.2020.05.002
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骨髓间充质干细胞促小鼠皮肤烫伤修复研究

Effect of bone marrow mesenchymal stem cells on recovery of skin burn in C57BL/6J mice

    摘要
  • 摘要:
    目的 探讨骨髓间充质干细胞(bone marrow mesenchymal stem cells，BMSCs)对小鼠皮肤烫伤的促修复作用，为临床采用BMSCs治疗烫伤提供依据。
    方法 以3周龄C57BL/6J小鼠的骨髓细胞悬液体外扩增培养BMSCs。将36只10周龄C57BL/6J小鼠随机分为局部细胞移植组、尾静脉细胞移植组和平行对照组(每组12只)，建立皮肤烫伤模型。小鼠皮肤烫伤损伤模型建立成功1 h后，将BMSCs通过皮下和尾静脉分别注射到两个细胞移植组中，平行对照组注射生理盐水。观察动物创面变化，计算21 d后的创面愈合率、检测炎症因子变化和皮肤形态学变化。
    结果 分离培养的BMSCs增殖旺盛，能够多向分化为成骨细胞、成软骨细胞和成脂细胞。局部细胞移植组和尾静脉细胞移植组的创面愈合率在21 d后达到98.2%和99.0%，其差异无统计学意义(P > 0.05)；而平行对照组愈合率在7 d后明显低于两个细胞移植组(P < 0.05)。BMSCs移植后，在第3天和第7天后，两个细胞移植组白细胞和白细胞介素-6(IL-6)水平明显低于平行对照组(P < 0.05)；在3 d、7 d和14 d后，两个细胞移植组C反应蛋白和肿瘤坏死因子-α(TNF-α)水平明显低于平行对照组(P < 0.05)；但21 d后各炎症因子水平在各组间差异无统计学意义(P > 0.05)。两个细胞移植组的皮肤形态学在21 d后胶原纤维丰富，皮肤组织结构清晰，而平行对照组可见炎性细胞浸润、胶原排列紊乱和充血现象。
    结论 皮肤烫伤模型移植BMSCs后，后者可提高宿主C57BL/6J小鼠的皮肤烫伤恢复能力。

     

    Abstract:
    Objective To study the effect of bone marrow mesenchymal stem cells(BMSCs) in promoting the recovery of burn wound in mice and provide experimental basis for the clinical treatment of BMSCs on skin burn.
    Methods BMSCs were cultivated and amplified from bone marrow cell suspension of 3-week-old C57BL/6J mice in vitro. Ten-week-old C57BL/6J mice(n=36)were used to establish the model of skin burn and then randomly divided into local cell transplantation group, tail vein cell transplantation group and control group. BMSCs were transplanted into the mice in cell transplantation groups by local injection and tail vein injection and saline was injected into the mice in control group, 1 h after establishment of skin burn mode. The changes of skin wounds, the wound healing rate, the changes of inflammatory factors and pathological changes were observed till 21 days.
    Results BMSCs had unlimited proliferation, and differentiated into osteoblasts, chondroblasts and lipoblasts. The wound healing rates of mice reached 98.2% and 99.0% at day 21 in the local cell transplantation group and the tail vein cell transplantation group, and there was no significant difference between the cell transplantation groups(P > 0.05). However, the wound healing rate of the control group was significantly lower than that of the two cell transplantation groups after 7 day(P < 0.05). After BMSCs transplantation, the levels of leucocyte and IL-6 in the cell transplantation groups were significantly lower than those in the control group at 3 and 5 days(P < 0.05). The levels of C-reactive protein and TNF-α in the cell transplantation groups were significantly lower than those in the control group at 3, 7 and 14 days(P < 0.05). However, there was no statistical difference in the levels of each inflammatory factor at 21 day(P > 0.05). In the two cell transplantation groups, the collagen fibers were abundant and skin tissue structure was clear at 21 days, while in the control group, inflammatory cell infiltration, collagen disorder and hyperemia were still observed at 21 days.
    Conclusion BMSCs can promote the recovery of skin burn wound in C57BL/6J mice.

     

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