Abstract: In view of the huge structural system of dangerous goods, the relatively scattered knowledge, and the complicated inquiry procedures and the difficult judgment of storage isolation, the method of constructing the knowledge graph of dangerous goods storage in the port was studied by taking the storage of dangerous goods in the ports as an example. Firstly, the knowledge structure of dangerous goods storage in ports was sorted out, and the dangerous goods were classified according to the characteristics of knowledge attributes (direct or indirect) and handling ways. Then, the concept, the entity, the attributes of dangerous goods, and the factual relationship among these goods were obtained. The ontology representation was used to represent the knowledge of goods and the relationship between the knowledge was established. On this basis, the knowledge graph of multi dangerous goods storage in ports was constructed. Finally, the application of the knowledge graph was discussed, and the effectiveness of the knowledge graph in simplifying the knowledge query procedure and storage isolation judgment was verified by a practical case.