杨家豪, 黄沪涛, 孙原, 赵乾魁, 王翔, 侯强, 张雪涛, 刘武忠, 孙品. 低浓度职业性苯乙烯接触对线粒体DNA拷贝数和微核率的影响研究[J]. 职业卫生与应急救援, 2022, 40(5): 517-523. DOI: 10.16369/j.oher.issn.1007-1326.2022.05.002
引用本文: 杨家豪, 黄沪涛, 孙原, 赵乾魁, 王翔, 侯强, 张雪涛, 刘武忠, 孙品. 低浓度职业性苯乙烯接触对线粒体DNA拷贝数和微核率的影响研究[J]. 职业卫生与应急救援, 2022, 40(5): 517-523. DOI: 10.16369/j.oher.issn.1007-1326.2022.05.002
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YANG Jiahao, HUANG Hutao, SUN Yuan, ZHAO Qiankui, WANG Xiang, HOU Qiang, ZHANG Xuetao, LIU Wuzhong, SUN Pin. Effects of occupationally low styrene exposure on workers' mitochondrial DNA copy number and micronucleus frequency[J]. Occupational Health and Emergency Rescue, 2022, 40(5): 517-523. DOI: 10.16369/j.oher.issn.1007-1326.2022.05.002
Citation: YANG Jiahao, HUANG Hutao, SUN Yuan, ZHAO Qiankui, WANG Xiang, HOU Qiang, ZHANG Xuetao, LIU Wuzhong, SUN Pin. Effects of occupationally low styrene exposure on workers' mitochondrial DNA copy number and micronucleus frequency[J]. Occupational Health and Emergency Rescue, 2022, 40(5): 517-523. DOI: 10.16369/j.oher.issn.1007-1326.2022.05.002
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低浓度职业性苯乙烯接触对线粒体DNA拷贝数和微核率的影响研究

Effects of occupationally low styrene exposure on workers' mitochondrial DNA copy number and micronucleus frequency

    摘要
  • 摘要:
      目的  探讨接触低浓度苯乙烯对线粒体DNA含量和微核率的影响，以寻找潜在的生物标志。
      方法  招募上海某塑料生产企业接触苯乙烯的男性工人127名为研究对象，检测工作车间空气中苯乙烯浓度，应用高效液相色谱法对苯乙烯的尿中代谢物进行检测，应用分光光度法测定尿中肌酐进行校正；按照尿中苯乙醇酸水平，将≤ 0.000 9 mg/g（以肌酐校正）者纳入低接触组，> 0.000 9~0.001 4 mg/g（以肌酐校正）者纳入中接触组，> 0.001 4 mg/g（以肌酐校正）者则纳入高接触组。通过实时荧光定量PCR实验检测工人外周血的线粒体DNA含量，通过胞质分裂阻滞微核实验检测外周血淋巴细胞的微核率。
      结果  工作场所定点采样显示环境空气中苯乙烯浓度低于1.2 mg/m3，尿中苯乙醇酸水平的中位数和四分位数为0.001 1（0.000 8，0.001 8）mg/g（以肌酐校正）。广义线性模型分析结果显示：与低接触组相比，中接触组工人的mtDNAcn（自然对数转换后）升高0.271（P=0.042）；中性粒细胞计数与淋巴细胞计数比值每升高1个单位，mtDNAcn（自然对数转换后）降低0.227（P < 0.01）。泊松回归模型分析结果显示：年龄每增加1岁，微核率升高至原来的1.043倍（P < 0.001）；尿中苯乙醇酸水平并非微核率的影响因素（P>0.05）。
      结论  即使是低于职业接触限值的苯乙烯接触亦可引起线粒体DNA拷贝数水平的改变，但未引起微核率的遗传毒性指标的改变，提示低浓度的苯乙烯可能通过氧化应激引起早期效应。

     

    Abstract:
      Objective  To investigate the adverse effect of styrene exposure at low concentrations on workers' mitochondrial DNA content and micronucleus frequency, in order to explore these as potential exposure biomarkers.
      Methods  Totally 127 male workers exposed to styrene in a plastic production enterprise in Shanghai were recruited as the study subjects. The concentration of styrene in the air of their workplaces was detected. High performance liquid chromatography was applied to detect urinary metabolites of styrene, and spectrophotometric method was applied to determine urinary creatinine for calibration purposes. The workers were divided into 3 groups, namely low, medium and high exposure, according to the urinary phenylglycolic acid levels, ≤ 0.000 9, 0.000 9 -0.001 4 and > 0.001 4 mg/g creatinine, respectively. Both mitochondrial DNA content and micronucleus frequency of peripheral blood were assessed by real-time fluorescence quantitative PCR assay and cytokinesis -block micronucleus assay, respectively.
      Results  Area sampling at workplaces revealed that ambient styrene concentrations were all below 1.2 mg/m3. The median and interquartile range of workers' urinary phenylglycolic acid levels were 0.001 1 (0.000 8, 0.001 8) mg/g creatinine. Generalized linear model analysis showed that mtDNAcn (after natural log transformation) was elevated by 0.271 (P = 0.042) in workers with medium exposure compared to the workers with low exposure； mtDNAcn (after natural log transformation) decreased by 0.227 (P = 0.002) with an increase in the ratio of neutrophil count to lymphocyte count. Poisson regression model analysis showed that the micronucleus rate increased to 1.043 times the original rate for each year of age (P < 0.001)；urinary phenylglycolic acid level was not an influential factor in the micronucleus rate (P > 0.05).
      Conclusions  Even exposure level was lower than the occupational exposure limit, styrene could still cause changes in mitochondrial copy number levels but not in the genotoxicity index of micronucleus frequency, which suggested that low concentrations of styrene may cause early effects through oxidative stress.

     

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