Abstract: Objective To appraise the effectiveness of exercise prescription on fatigue-predominant sub-health of students. Methods With stratified randomly cluster sampling,a total of 761 undergraduates from different grades in a university were sampled and their fatigue status were self-estimated with a self-designed fatigue-related questionnaire.Then 269 students with fatigue-predominant sub-health were divided into 2 groups,135 of them in intervention group, who received exercise prescription and 134 in control group, who received no intervention measures. Results The baseline survey showed that the prevalence rate of fatigue-predominant sub-health among the students was 35.35%. it showed that there was statistical difference between different grades in the prevalence rates of fatigue-predominant sub-health(χ²=28.257,P<0.01). The second survey after 3 months' exercise prescription intervention showed that the improvement rate was 87.4% and 38.1% in intervention and control groups respectively(χ²=70.119,P<0.01). Conclusion The exercise prescription intervention had remarkable effect on improvement of students' fatigue-predominant sub-health.